Human Carbonic Anhydrases

Abstract

Abstract Sequence studies of human erythrocyte carbonic anhydrase B show that it contains 256 residues. A series of relatively small peptides soluble in aqueous solutions obtained by chymotryptic and tryptic digestion of the enzyme, large peptides containing only COOH-terminal lysine or arginine as well as those resulting from more limited cleavage of the enzyme, and cyanogen bromide cleavage products served as the materials for deriving the primary structure.