Synthesis of Ovalbumin in a Rabbit Reticulocyte Cell-free System Programmed with Hen Oviduct Ribonucleic Acid

Abstract

Hen oviduct RNA can direct the synthesis of the major oviduct protein, ovalbumin, in a cell-free system derived from rabbit reticulocytes. The protein was identified by precipitation with anti-ovalbumin, migration on gel electrophoresis, and ion exchange chromatography of tryptic peptides. Ovalbumin messenger RNA activity appears to sediment in a sucrose gradient on the low molecular weight side of the 18 S ribosomal RNA.