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A monoclonal antibody (BA-1) reactive with cells of human B lymphocyte lineage.
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1981
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Lymphocyte DevelopmentMixed-phenotype Acute LeukemiaImmunologyPathologyImmune SystemImmunotherapyMyeloid NeoplasiaHematological MalignancyWeak ReactivityHematologyAntibody EngineeringLymphocyte BiologyMonoclonal AntibodyLymphoid NeoplasiaAutoimmunitySurface ImmunoglobulinCell BiologyAntibody BiologyMalignant Blood DisorderMedicineImmune Cell Activation
NALM‑6‑M1 is an acute lymphoblastic leukemia cell line that expresses a pre‑B lymphocyte phenotype (cytoplasmic IgM⁺, surface immunoglobulin‑). Hybridomas were generated by fusing spleen cells from hyperimmunized mice with NS‑1 mouse myeloma cells. BA‑1 binds peripheral blood B lymphocytes, chronic lymphocytic leukemia, pre‑B‑ALL, most non‑Hodgkin lymphomas, and non‑T, non‑B ALL, shows weak reactivity with B‑lymphoblastoid lines, does not react with multiple myeloma, plasma cells, T cells, platelets, red cells, monocytes, or AML, reacts with granulocytes and the K‑562 erythroleukemia line, and its epitope is distinct from surface Ig, HLA‑DR, or C3/Fc receptors, indicating its potential use in studying early human B‑cell development.
NALM-6-M1 is an acute lymphoblastic leukemia cell line previously shown in our laboratory to express the pre-B lymphocyte phenotype, i.e., cytoplasmic IgM+, surface immunoglobulin-. Hybridomas were produced against this cell line by fusing spleen cells from hyperimmunized mice with NS-1 mouse myeloma cells. One monoclonal antibody derived from this fusion, designated BA-1, reacted with peripheral blood B lymphocytes, chronic lymphocytic leukemias, pre-B-ALL, most non-Hodgkin's lymphomas, and most non-T, non-B-ALL. BA-1 showed weak reactivity with B lymphoblastoid cell lines and failed to react with multiple myeloma and pokeweed mitogen-induced plasma cells. BA-1 also reacted with peripheral blood granulocytes and the erythroleukemia cell line K-562. No reactivity was seen with cells of T lymphocyte origin, platelets, red cells, monocytes, or acute myelocytic leukemias. Evidence is presented indicating that the determinant recognized by BA-1 is not surface immunoglobulin, HLA-DR, or receptors for C3 or Fc. We conclude that monoclonal antibody BA-1 may be useful in the study of early stages of human B lymphocyte development.