Publication | Open Access
Restriction and Modification in Bacillus subtilis. Localization of the Methylated Nucleotide in the BsuRI Recognition Sequence
55
Citations
9
References
1978
Year
Dc DcDnaGeneticsDna MethylationBacteriologyDna AnalysisMolecular BiologyMolecular GeneticsGenomicsEpigeneticsCalf Thymus DnaBacillus SubtilisNucleic Acid ChemistryDna SequencingBiochemistryOligonucleotideDna ReplicationBsuri Recognition SequenceDna DemethylationMolecular MicrobiologyChromatinNatural SciencesMicrobiologyMethylated NucleotideMedicineMicrobial Genetics
Calf thymus DNA was methylated in vitro with cell extracts of Bacillus subtilis OG3R (r+m+) and S-adenosyl[Me-3H]methionine. After depurination of the [3H]methylated DNA, the analysis of the pyrimidine dinucleotides revealed the following positions of the methylated nucleosides (indicated by an asterisk) within the BsuRI recognition sequence: 5' dG--dG--dC--dC dC--dC--dG--dG 5'.
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