Electroanalysis · 2015 · 25 citations · 36 references
Natural SciencesChemistryPharmaceutical AnalysisAnalytical ChemistryElectroanalytical MethodsElectrochemistryBioanalytical Method ValidationElectroanalytical ChemistryElectrochemical DetectionElectrode MaterialsElectrolyte SolutionsInstrumental AnalysisAdsorptive Stripping VoltammetryDifferential Pulse VoltammetryElectrolyte CompositionMethod Detection LimitPreconcentrationRepeatabilityVoltammetric DetectionVoltammetry
Abstract Fast and sensitive method of prednisolone determination at renewable mercury film electrode using differential pulse adsorptive stripping voltammetry (DPAdSV) was successfully developed. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and supporting electrolyte composition were optimized. A linear voltammetric response for analyte was obtained in the concentration range from 0.05 µmol L −1 (18 µg L −1 ) to 2.25 µmol L −1 (0.81 mg L −1 ), with a detection limit of 0.01 µmol L −1 for preconcentration time of 20 s. Repeatability of method was determined as RSD % for prednisolone concentration of 0.04 µmol L −1 as 1.6 % (n=9). The proposed method was successfully applied and validated by recovery parameter of prednisolone in simulated sample and pharmaceutical product.
36
Electrochemical methods, fundamentals and applications
D.A. Aikens · Journal of Chemical Education · 1983 · 3.2K citations