Chemopreventive and antilipidperoxidative potential of thespesia populnea (L.) on experimental buccal pouch carcinogenesis

Abstract

The present study has investigated the chemopreventive potential and antilipidperoxidative effect of ethanolic extract of Thespesia populnea (L.)bark on 7,12-dimethylbenz[a] anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral squamous cell carcinoma had developed in Syrian golden hamsters by exposing the buccal mucosa to 0.5 % DMBA in liquid paraffin thrice a week for 14 weeks. The tumor incidence, tumor volume and tumor burden that occurred in the hamster buccal pouch were determined. Oral administration of ethanolic extract of T.populnea bark at a dose of 300 mg/kg b.wt to DMBA painted animals on alternate days of DMBA treated for 14 weeks significantly prevented the tumor incidence, tumor volume and tumor burden. Ethanolic extract of T.populnea bark showed potent antilipidperoxidative effect as well as enhanced the antioxidants status in DMBA painted animals. We thus conclude that ethanolic extract of T.populnea bark has potent chemopreventive efficacy and significant antilipidperoxidative effect in DMBA induced oral carcinogenesis. Further studies are needed to isolate and characterize the bioactive chemopreventive component from the bark of T.populnea.