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H-2 COMPONENTS AND CELLULAR MEMBRANES
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1967
Year
HistocompatibilityImmunohematologyIsoantigenic VariantsImmunologyPathologyImmunophenotypingImmune SystemPlasma Membrane FractionRedox BiologyCellular PhysiologyTumor BiologyMembrane TransportSurgical PathologyHematologyMolecular PathologySoluble FractionMolecular OncologyHealth SciencesLymphoid NeoplasiaHistopathologyImmune SurveillanceMembrane SystemMembrane PermeationCell BiologyCellular BiochemistryMedicine
Two isoantigenic variants of an A/Sn X A.SW lymphoma (LNSF) were separated into the following subcellular fractions: soluble material, total subcellular particulates, “microsomes”, plasma membrane and endoplasmic reticulm. The distribution in these fractions of 6 H-2 specificities was studied using monospecific or nearly monospecific antisera. Hemagglutination inhibition tests were used to detect soluble antigens. Absorption of hemagglutinating antibodies was employed to detect H-2 specificities in particulate fractions. All of the H-2 specificities known to be present in the tumors were analyzed with respect to their subcellular distribution. H-2.19, H-2.3 and H-2.8 were found to be represented in the plasma membrane fraction but could not be detected on endoplasmic reticulum. H-2.5 was found to be soluble. Some H-2.19 was also located in the soluble fraction. H-2.4 was present in the microsomes and H-2.23, a specificity possibly synonymous with the abandoned H-2.W, was represented in both plasma membrane and endoplasmic reticulum.