Cellulase production in continuous and fed-batch culture by Trichoderma reesei MCG80

Abstract

Continuous culture of Natick's strain MCG80 of Trichoderma reesei at a dilution rate of 0.028 h/sup -1/ has yielded a cellulase titer of over 61 U/mL using 5% lactose as the sole carbon source. Enzyme productivity at this dilution rate is 168 IU/L/h. Repeated fed-batch cultures using this strain on lactose as the carbon source have titers of 10 IU/mL with productivities in excess of 100 IU/L/h. 5 figures, 1 table.