Mixed Function Oxidation

Abstract

The isolation of a pure reduced diphosphopyridine nucleotide:(acceptor) oxidoreductase from Pseudomonas putida, strain CIB, in 15% yield is described.2. The absorption spectrum, fluorescence spectrum, and lack of activity with dye electron acceptors show the isolated enzyme to be devoid of flavin (apoenzyme).Iron, copper, and molybdenum are also absent.,.