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A Plaque Assay on Agar for Detection of Gram-Negative Bacilli Sensitive to Complement
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1972
Year
ImmunohematologyPlaque AssayPathogenic MicrobiologyPathogen DetectionSerum ResistantSerum Sensitive BacteriaMedical MicrobiologyBioanalysisInfection ControlAntimicrobial ResistanceHealth SciencesAllergyClinical MicrobiologyComplement SystemGram-negative BacteriologyAntimicrobial SusceptibilityMicrobial DiseaseGram-negative Bacilli SensitivePathogenesisMicrobiologyMedicineNormal SerumDiagnostic Microbiology
Summary A screening method to detect serum (complement) sensitive Gram-negative bacilli is described. Normal serum, dropped on the surface of a T.S.A. plate inoculated with the test organism will inhibit the growth of serum sensitive bacteria. Gram-negative bacteria isolated from blood cultures were tested and only 9/120 were sensitive. Bacteria isolated from infections, other than urinary tract, were also usually serum resistant.