Journal of Bacteriology · 1995 · 91 citations · 29 references
Life SciencesGeneticsMolecular GeneticsGenome OrganizationGenomicsMicrobial GeneticsChromosome BiologyChromosome MappingGene MappingPlasmid BiologyPlasmid GeneticsRestriction Enzyme TechnologyGel ElectrophoresisPlasmid Biology In FungiPulsed-field Gel ElectrophoresisRestriction EndonucleasesRestriction Fragment Analysis
The chromosomal DNA of Streptomyces griseus 2247 (a derivative of strain IFO3237) was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestion with AseI and DraI gave 15 and 9 fragments, respectively, the total sizes of which were 7.8 Mb. All the AseI and DraI fragments were aligned on a linear chromosome map by using linking plasmids and cosmids. PFGE analysis of the intact chromosome also showed a linear DNA band of about 8 Mb. Detailed physical maps of both terminal regions were constructed; they revealed the presence of a 24-kb terminal inverted repeat on each end. PFGE analysis with and without proteinase K treatment suggested that each end of the chromosome carries a protein molecule.
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The chromosomal DNA of <i>Streptomyces lividans</i> 66 is linear
Yi‐Shing Lin, Helen M. Kieser, David A. Hopwood et al. · Molecular Microbiology · 1993 · 277 citations