The Journal of Immunology · 1985 · 109 citations · 0 references
Life SciencesBiochemistryImmunologyHematology PhysiologyInflammationInnate ImmunityInflammatory MediatorsLeukocyte RecruitmentNeutrophil BiologyPlatelet ActivationGas Chromatography-mass SpectrometryNeutrophil Effector FunctionsNeutrophil MetabolismNeutrophil PhysiologyNeutrophil Signaling PathwaysNeutrophil-mediated InflammationPlatelet-activating Factor SignalingSelected Ion Monitoring
Two species of platelet-activating factor (PAF), 1-hexadecyl- and 1-octadecyl-2-acetyl-sn-glycero-3-phosphocholine (C16 = 0 AGEPC and C18 = 0 AGEPC) were detected in ionophore A23187-stimulated human neutrophils. The amount of AGEPC in 1 x 10(7) neutrophil cells was 80 +/- 26 pmol (mean +/- standard error) with a range of 14 to 223 pmol (n = 8), and it consisted of 80% of the C16 = 0 species and 20% of the C18 = 0 species. Most of the AGEPC derived from ionophore-treated neutrophils remained cell associated rather than being secreted into the medium, even when the medium contained ample albumin protein, which can trap AGEPC. These results were obtained by a technique of gas chromatography-mass spectrometry coupled with selected ion monitoring.