Publication | Closed Access
DNA-Directed Functionalization of Colloidal Gold with Proteins This work was supported by Deutsche Forschungsgemeinschaft and Fonds der Chemischen Industrie. We thank Prof. D. Blohm for helpful discussions and generous support.
196
Citations
0
References
2001
Year
NanoparticlesDna-functionalized Gold NanoparticlesEngineeringGenerous SupportMolecular BiologyBiomedical EngineeringChemical BiologyProtein NanoparticlesNanomedicineDna-directed FunctionalizationDna NanotechnologySupramolecular Construction KitAntibody EngineeringBioimagingDna ComputingBiological NanomaterialsBiochemistryBiomedicineNanobiotechnologyBioconjugationDna-stabilized Colloidal GoldMolecular EngineeringBiomolecular EngineeringColloidal GoldBiomedical DiagnosticsNatural SciencesSynthetic BiologyProtein Engineering
A supramolecular construction kit comprises DNA-functionalized gold nanoparticles, covalent DNA–streptavidin conjugates, and biotinylated antibodies and allows the generation of functional nanoparticle reagents (see schematic illustration). The combination of specific antibodies and DNA-stabilized colloidal gold is applicable for the detection of antigens attached to a chip.