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A rapid method for the construction of synthetic genes using the polymerase chain reaction.
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1990
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Viral ReplicationEngineeringGeneticsMolecular BiologyNucleic Acid Amplification TestSynthetic CircuitRapid MethodReal-time Polymerase Chain ReactionTransgenic TechnologyPolymerase Chain ReactionGenetic TechnologyHuman RetrovirusDans Cet ArticleGenome EngineeringProteine RevSynthetic GenesDirected EvolutionDna ReplicationVirologyHivBiomolecular EngineeringSynthetic BiologyGenetic EngineeringNucleic Acid AmplificationMedicineDecrivent Un Protocole
Dans cet article, les auteurs decrivent un protocole de PCR en 2 etapes, utilisant la taq polymerase thermostable, pour creer un gene synthetique pour la proteine Rev de HIV2