Assignment of the Human Casein Kinase II α′ Subunit Gene (CSNK2A1) to Chromosome 16p13.2-p13.3

Abstract

The authors have previously mapped the CK II-[beta] gene (CSNK2B) to chromosome 6p12-p21 and the CK II-[alpha] sequence to two sites, chromosomes 11p15.5-p15.4 and 20p13, the latter having been verified by other investigators. The sequencing of a genomic human DNA fragment has shown that the CK II-[alpha] gene (CSNK2A) localized to chromosome 11 is a processed (pseudo) gene and therefore the active gene is presumably on chromosome 20. The other catalytic subunit gene CK II-[alpha][prime] was localized to chromosome 16 by somatic cell hybrid analysis. The authors now report the regional mapping of the CK II-[alpha][prime] gene (CSNK2A1) to chromosome 16p13.2-p13.3. The probe used was a 414-bp fragment from the 3[prime] nontranslated region of the human CK II-[alpha][prime] cDNA. Chromosomal localization was carried out by in situ hybridization as previously described. Of 128 grains scored in 75 cells, 13 (10.2%) were located on the distal short arm of chromosome 16, bands p13.2-p13.3. No other sites were labeled above background. 7 refs., 1 fig.