Enzymic synthesis of glycosides of racemic alcohols using .BETA.-galactosidase and separation of the diastereomers by high-performance liquid chromatography using a conventional column.

Abstract

β-Glycosides of phenylethylene glycol, propylene glycol, 3-chloropropylene glycol and 1-phenylethyl alcohol were synthesized by transglycosidation using β-galactosidase from Aspergillus oryzae, and the diastereomers were resolved by high-performance liquid chromatography on a conventional column.