Genetic Analysis of Transfer by the <i>Escherichia coli</i> Sex Factor F, Using P1 Transductional Complementation

Abstract

P1 transduction has been used to perform a complementation analysis of a series of transfer-deficient mutants of Flac. The results define ten cistrons and are consistent with the results of a conjugational analysis presented in an accompanying report. Both sets of results are summarized here. Between them, they define eleven cistrons, traA through traK, necessary for conjugational deoxyribonucleic acid (DNA) transfer. Mutants in traI and traD and some in traG still make F-pili, although traD mutants are resistant to f2 phage; their products may be involved in conjugational DNA metabolism. Other mutants in traG and all mutants in the remaining eight cistrons do not make F-pili. One of these, traJ, may be a control cistron, and the others may specify a biosynthetic pathway responsible for synthesis and modification of the F-pilin subunit protein and its assembly into the F-pilus.