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STUDIES OF THE IMMUNE GLOBULINS OF HUMAN SERUM. I. A METHOD FOR THE SIMULTANEOUS ISOLATION OF THE TREE IMMUNE GLOBULINS (GAMMA-SS, GAMMA-IM AND GAMMA-1A) FROM INDIVIDUAL SMALL SERUM SAMPLES.
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1963
Year
ImmunohematologyLaboratory ImmunologyImmunodeficienciesImmunologyGamma-im And Gamma-1aPathologyImmunodominanceImmune Globulin FractionsProtein PurificationBioanalysisHematologySerologic TestingImmunochemistryImmune GlobulinsClinical ChemistryChromatographyHealth SciencesAutoimmune DiseaseAllergyAutoimmunityHumoral ImmunityThe Simultaneous IsolationImmune FunctionPseudoglobulin SolutionAntibody ScreeningAntibody BiologyImmunoglobulin EMedicine
Summary A method is described which allows the isolation of antigenically pure samples of the three immune globulins: γ ss (=7 S γ), γ 1M (=19 S γ) and γ 1A (=β 2A ), starting from individual serum samples. The γ ss -globulin is obtained by chromatography on diethylaminoethyl-cellulose. Preparative ultracentrifugation in a sucrose density gradient is used to obtain the γ 1M -globulin from a euglobulin precipitate. The γ 1A -globulin is isolated from the remaining pseudoglobulin solution, by means of a modified fractionation procedure using precipitation with zine sulfate, preparative electrophoresis and salting out with ammonium sulfate, in the order indicated. The purity and recovery rates of the three immune globulin fractions are assessed, and their value as representatives of the corresponding immune globulin groups of native serum is critically discussed.