European Journal of Biochemistry · 2003 · 25 citations · 19 references
Life SciencesBiochemistryPlant SciencesMetabolismNatural Product BiosynthesisPlant BiochemistryPhenylpropanoid BiosynthesisPhenylpropanoid MetabolismPlant Natural Product BiosynthesisPolyketide BiosynthesisSecondary Metabolite BiosynthesisShikimate PathwayAromatic Compound BiosynthesisCinnamic Acid MetabolismIsotopomer AnalysisPhenylalanine BiosynthesisPlant Shikimate PathwayQuantitative Nmr SpectroscopyShikimate BiosynthesisShikimic Acid
Root cultures of Swertia chirata (Gentianaceae) were grown with supplements of [1-13C]glucose, [U-13C6]glucose or [carboxy-13C]shikimic acid. 1,3,5,8-Tetrahydroxyxanthone was isolated and analysed by quantitative NMR analysis. The observed isotopomer distribution shows that 1,3,5,8-tetrahydroxyxanthone is biosynthesized via a polyketide-type pathway. The starter unit, 3-hydroxybenzoyl-CoA, is obtained from an early shikimate pathway intermediate. Phenylalanine, cinnamic acid and benzoic acid were ruled out as intermediates.
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