ManR, a Transcriptional Regulator of the β-Mannan Utilization System, Controls the Cellulose Utilization System in <i>Aspergillus oryzae</i>

Abstract

ManR, a DNA-binding protein possessing the Zn(II)(2)Cys(6) binuclear cluster domain, acts as a positive regulator of mannanolytic enzyme genes in Aspergillus oryzae. In this study, we found that ManR controlled the expression not only of mannanolytic enzyme genes but also of cellulolytic enzyme genes in A. oryzae, based on DNA microarray analysis of a manR-disruptant and a manR-overexpressing strain. A new model for the regulation of cellulase and hemicellulase genes mediated by ManR and XlnR in A. oryzae is proposed.