The Specificity of Formolized Proteins

Abstract

Summary The serological specificity of formolized serum-proteins has been restudied with a new sort of preparation and extensive controls. The specific alterations following formolization can be demonstrated only in certain reactions occurring between antigens and antisera from related species, resembling alterations produced by heating proteins rather than the more marked changes following halogenation or nitration. A comparison of formolized antigens and antisera prepared by different methods suggests that secondary changes leading to gelatinization play an important rôle. Very faint traces of turbidity in test antigens may be associated with falsely positive reactions.