High Cellobiase and Xylanase Production by <i>Sclerotium rolfsii</i> UV-8 Mutant in Submerged Culture

Abstract

Sclerotium rolfsii UV-8 mutant secretes high levels of cellobiase and xylanase in addition to having high cellulase production. The apparent K(m) and V(max) of cellobiase (grown in NM-2 + 2% corn steep liquor medium) with cellobiose as a substrate were 5.6 mM and 22.2 mumol of glucose liberated per min per ml of culture filtrate, respectively. The addition of 2% corn steep liquor to NM-2 medium increased endo-beta-glucanase, cellobiase, and xylanase yields by approximately 1.5-fold.