Identification of <i>Anguilla anguilla</i> (L.) and <i>Anguilla rostrata</i> (Le Sueur) and their hybrids based on a diagnostic single nucleotide polymorphism in nuclear 18S rDNA

Abstract

The variability of the nuclear 18S rDNA was examined for 10 species of freshwater eels: Anguilla anguilla, A. australis, A. dieffenbachii, A. japonica, A. marmorata, A. mossambica, A. nebulosa labiata, A. obscura, A. reinhardtii and A. rostrata. We observed that a single nucleotide polymorphism (SNP) separated A. anguilla and A. japonica from the remaining taxa. While this SNP marker may be used to identify hypothetical hybrids of A. japonica and sympatric eel species, we focused on Atlantic eels to evaluate the potential for this diagnostic chromosomal marker to discriminate between individuals of A. anguilla, A. rostrata and their hybrids.