Genomics Proteomics & Bioinformatics · 2003 · 19 citations · 9 references
Life SciencesMolecular BiologyMolecular GeneticsGenomicsMolecular VirologyVirologyEmerging Viral DiseasesViral EvolutionViral GeneticsViral GenomicsViral InfectionsHost-virus CoevolutionVariant Emergence StudiesViral Genome EvolutionViral Transmission DynamicsVirus GenotypesVirus-host CoevolutionVirus-host DynamicsVirus-host Interaction Analysis
We report a complete genomic sequence of rare isolates (minor genotype) of the SARS-CoV from SARS patients in Guangdong, China, where the first few cases emerged. The most striking discovery from the isolate is an extra 29-nucleotide sequence located at the nucleotide positions between 27,863 and 27,864 (referred to the complete sequence of BJ01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream of the N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29, suggests a significant genetic event and differentiates it from the previously reported genotype, the dominant form among all sequenced SARS-CoV isolates. A 17-nt segment of this extra sequence is identical to a segment of the same size in two human mRNA sequences that may interfere with viral replication and transcription in the cytosol of the infected cells. It provides a new avenue for the exploration of the virus-host interaction in viral evolution, host pathogenesis, and vaccine development.
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Coronavirus as a possible cause of severe acute respiratory syndrome
ST Lai, Yi Guan, Lyc Yam et al. · The Lancet · 2003 · 3K citations · Full text
Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome
Paul A. Rota, M. Steven Oberste, Stephan S. Monroe et al. · Science · 2003 · 2.6K citations · Full text
The Genome Sequence of the SARS-Associated Coronavirus
Marco A. Marra, Steven J.M. Jones, Caroline R. Astell et al. · Science · 2003 · 2.1K citations