CHROMOGENIC SUBSTRATES

Abstract

This paper describes the use of p-nitrophenyl sulfate as a chromogenic substrate for the calorimetric assay of the activity of phenolsulfatase. In neutral or alkaline solution the substrate is colorless; on enzymatic hydrolysis p-nitrophenol is liberated, which at alkaline pH develops a yellow color suitable for the quantitative measurement of activity of the enzyme. Advantages which this method holds over earlier procedures include greater simplicity, accuracy, and sensitivity.