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Inhibition of chemiluminescence response of human mononuclear cells and suppression of mitogen-induced proliferation of spleen lymphocytes of mice by hispolon and hispidin.
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1996
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Chemiluminescence ResponseImmunologyCellular PhysiologyPolyphenolicsInflammationBioanalysisHematologyLymphatic SystemMitogen-induced ProliferationToxicologyPhytopharmacologyClinical ChemistryPhytochemicalCell SignalingInhibitory ActivityBiochemistryExperimental ToxicologyPharmacologyCell BiologyPhagocytePhenolic CompoundsHuman Mononuclear CellsPhytochemistryMedicine
The effects of two phenolic compounds, hispolon and hispidin isolated from fruit bodies of the basidiomycete Inonotus hispidus (Bull. ex Fr.) Karst, were investigated on the chemiluminescence response by LPS- or zymosan-activated human mononuclear cells (MNC) and on the concanavalin A-induced proliferation of spleen lymphocytes of mouse in vitro. Both compounds showed inhibitory activity in the chemiluminescence-test with an IC50 (the concentration of test compound causing 50% effect) ranging from 4.4 to 4.6 micrograms/ml (20.3 to 21.2 microM) for hispolon and < 0.1 to 1.5 micrograms/ml (from < 0.4 microM to 6.0 microM) for hispidin. Antiproliferative effects have been achieved in the lymphocyte transformation test (LTT) by hispolon with an IC50 of 3.4 micrograms/ml (15.5 microM).