Differential Staining of Parental Chromosomes in Interspecific Cell Hybrids with a Combined Quinacrine and 33258 Hoechst Technique

Abstract

A simple method is described of differential staining of parental chromosomes in human-mouse, dog-mouse, and rat-mouse hybrid cells, with a mixed solution of QM and 33258 Hoechst. This technique allows not only the discrimination of parental complements by bright centromeric bands specific for the mouse chromosomes, but also simultaneous identification of individual chromosomes by their characteristic Q-band patterns.