Concepedia

Temperature-dependent Unfolding

Temperature-Dependent Unfolding is a biophysical assay method that measures the thermal denaturation profile of a biomolecule, particularly proteins or nucleic acids, by monitoring changes in circular dichroism signal as a function of temperature to determine structural stability and unfolding transitions.

357

Publications

20.7K

Citations

1.2K

Authors

448

Institutions

Publications per year

2017–2026

32

Variants

Thermal DenaturationThermal Unfolding

Parents

Publications

357

Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions

Norma J. Greenfield · Nature Protocols · 2006 · 902 citations · Full text

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Mechanism of Helix Induction by Trifluoroethanol:  A Framework for Extrapolating the Helix-Forming Properties of Peptides from Trifluoroethanol/Water Mixtures Back to Water

Peizhi Luo, Robert L. Baldwin · Biochemistry · 1997 · 653 citations

, , +17

Thermal difference spectra: a specific signature for nucleic acid structures

Jean‐Louis Mergny · Nucleic Acids Research · 2005 · 456 citations · Full text

, , +16

Unfolding proteins by external forces and temperature: The importance of topology and energetics

Emanuele Paci, Martin Karplus · Proceedings of the National Academy of Sciences · 2000 · 300 citations

, , +17

The molecular mechanism of thermal unfolding of Escherichia coli formylmethionine transfer RNA

Donald M. Crothers, Patricia E. Cole, Cornelis W. Hilbers et al. · Journal of Molecular Biology · 1974 · 286 citations

, , +16

1–5 of 357

Authors

1.2K

  1. RW
    Roland Winter

    TU Dortmund University, Germany

    6 publications · 565 citations

    6

    h-index

  2. GI
    George I. Makhatadze

    Rensselaer Polytechnic Institute, United States

    5 publications · 648 citations

    5

    h-index

  3. HA
    Harold A. Scheraga

    Cornell University, United States

    5 publications · 225 citations

    5

    h-index

  4. JS
    Joachim Seelig

    University of Basel, Switzerland

    5 publications · 145 citations

    5

    h-index

  5. DM
    Danilo Milardi

    University of Catania, Italy

    4 publications · 91 citations

    4

    h-index

1–5 of 1.2K

Institutions

448

  1. Centre National de la Recherche Scientifique

    Paris, France

    13 h-index

    52 publications

    5K citations

  2. Johns Hopkins University

    Baltimore, United States

    8 h-index

    22 publications

    1.9K citations

  3. University of Basel

    Basel, Switzerland

    6 h-index

    11 publications

    303 citations

  4. Yale University

    New Haven, United States

    6 h-index

    20 publications

    3K citations

  5. KU Leuven

    Leuven, Belgium

    6 h-index

    15 publications

    913 citations

  6. Massachusetts Institute of Technology

    Cambridge, United States

    5 h-index

    13 publications

    1.3K citations

  7. Max Delbrück Center

    Berlin, Germany

    5 h-index

    13 publications

    653 citations

  8. Amgen (United States)

    Thousand Oaks, United States

    5 h-index

    24 publications

    644 citations

1–8 of 448

Venues

  1. Biochemistry

    66 publications · 4.6K citations

    36

    h-index

  2. Journal of Biological Chemistry

    JBC · 19 publications · 1.5K citations

    16

    h-index

  3. Proceedings of the National Academy of Sciences

    PNAS · 16 publications · 1.9K citations

    16

    h-index

  4. Journal of Molecular Biology

    JMB · 15 publications · 1.6K citations

    14

    h-index

  5. The Journal of Physical Chemistry B

    14 publications · 465 citations

    13

    h-index

1–5