Concepedia

Second-strand Complementary Dna Synthesis

Second-Strand Complementary DNA Synthesis is the enzymatic process in molecular biology whereby the second strand of complementary DNA is synthesized from a single-stranded cDNA template, typically using DNA polymerase, to generate double-stranded cDNA for use in cDNA library construction.

19

Publications

1.4K

Citations

89

Authors

42

Institutions

Publications per year

2017–2026

3

Variants

Second-strand Cdna Synthesis

Parents

Publications

19

Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.

Marvin Wickens, Gary Buell, Robert Schimke · Journal of Biological Chemistry · 1978 · 395 citations · Full text

, , +14

Second-Strand Synthesis-Based Massively Parallel scRNA-Seq Reveals Cellular States and Molecular Features of Human Inflammatory Skin Pathologies

Marc H. Wadsworth, Todd M. Gierahn, Tran Do et al. · Immunity · 2020 · 277 citations · Full text

, , +15

RNA sequencing by direct tagmentation of RNA/DNA hybrids

Lin Di, Yusi Fu, Yue Sun et al. · Proceedings of the National Academy of Sciences · 2020 · 143 citations · Full text

, , +11

The tymobox, a sequence shared by most tymoviruses: its use in molecular studies of tymoviruses

Shou‐Wei Ding, Jennifer Howe, Paul Keese et al. · Nucleic Acids Research · 1990 · 103 citations · Full text

, , +17

A putative primer for second‐strand DNA synthesis of maize streak virus is virion‐associated.

Jonathan Donson, Bret A.M. Morris-Krsinich, Philip M. Mullineaux et al. · The EMBO Journal · 1984 · 98 citations · Full text

, , +12

1–5 of 19

Authors

89

  1. DW
    Dong Wang

    University of California San Diego, United States

    2 publications · 154 citations

    2

    h-index

  2. JX
    Jun Xu

    University of California San Diego, United States

    2 publications · 154 citations

    2

    h-index

  3. LD
    Lin Di

    Peking University, China

    2 publications · 154 citations

    2

    h-index

  4. YH
    Yanyi Huang

    Peking University, China

    2 publications · 154 citations

    2

    h-index

  5. RW
    Raymond W. Lee

    Washington State University, United States

    2 publications · 154 citations

    2

    h-index

1–5 of 89

Institutions

42

  1. University of California, Los Angeles

    Los Angeles, United States

    2 h-index

    10 publications

    1.6K citations

  2. Shenzhen Bay Laboratory

    Shenzhen, China

    2 h-index

    5 publications

    319 citations

  3. Peking University

    Beijing, China

    2 h-index

    10 publications

    770 citations

  4. Center for Life Sciences

    Beijing, China

    2 h-index

    13 publications

    671 citations

  5. Nanjing Normal University

    Nanjing, China

    2 h-index

    2 publications

    154 citations

  6. Tsinghua University

    Beijing, China

    2 h-index

    8 publications

    616 citations

  7. Institute of Structural and Molecular Biology

    London, United Kingdom

    1 h-index

    5 publications

    70 citations

  8. Boston Children's Hospital

    Boston, United States

    1 h-index

    2 publications

    554 citations

1–8 of 42

Venues

  1. Nucleic Acids Research

    4 publications · 255 citations

    4

    h-index

  2. Phytopathology

    2 publications · 104 citations

    2

    h-index

  3. Journal of Virology

    JVI · 2 publications · 32 citations

    2

    h-index

  4. Journal of Molecular Biology

    JMB · 2 publications · 59 citations

    2

    h-index

  5. bioRxiv (Cold Spring Harbor Laboratory)

    Repository · 1 publications · 11 citations

    1

    h-index

1–5