Concepedia

Engineered Polymerases

Engineered Polymerases is a laboratory-modified enzyme derived from natural DNA or RNA polymerases that has been structurally and functionally optimized through rational design or directed evolution to selectively recognize and replicate non-canonical nucleic acid substrates, such as xenonucleic acids, thereby enabling the expansion of the genetic alphabet and the implementation of orthogonal information storage and retrieval systems.

81

Publications

5.6K

Citations

309

Authors

114

Institutions

Publications per year

2017–2026

28

Parents

Publications

81

A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides.

Stanley Tabor, C C Richardson · Proceedings of the National Academy of Sciences · 1995 · 352 citations

, , +14

A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro

Yonghui Wang · Nucleic Acids Research · 2004 · 314 citations · Full text

, , +15

Amplification, Mutation, and Sequencing of a Six-Letter Synthetic Genetic System

Zunyi Yang, Fei Chen, J. Brian Alvarado et al. · Journal of the American Chemical Society · 2011 · 297 citations

, , +11

An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts

Athanasios Dousis, Kanchana Ravichandran, Elissa M. Hobert et al. · Nature Biotechnology · 2022 · 221 citations · Full text

, , +14

Modular control of multiple pathways using engineered orthogonal T7 polymerases

Karsten Temme, Rena M. Hill, Thomas H. Segall-Shapiro et al. · Nucleic Acids Research · 2012 · 198 citations · Full text

, , +17

1–5 of 81

Authors

309

  1. JC
    John C. Chaput

    University of California, Irvine, United States

    14 publications · 1.1K citations

    14

    h-index

  2. MR
    Matthew R. Dunn

    University of California, Irvine, United States

    8 publications · 623 citations

    8

    h-index

  3. FE
    Floyd E. Romesberg

    Scripps Research Institute, United States

    6 publications · 456 citations

    6

    h-index

  4. SA
    Steven A. Benner

    Foundation for Applied Molecular Evolution, United States

    5 publications · 449 citations

    5

    h-index

  5. PH
    Philipp Holliger

    MRC Laboratory of Molecular Biology, United Kingdom

    3 publications · 129 citations

    3

    h-index

1–5 of 309

Institutions

114

  1. University of California, Irvine

    Irvine, United States

    11 h-index

    36 publications

    2.6K citations

  2. Scripps Research Institute

    San Diego, United States

    6 h-index

    21 publications

    1.9K citations

  3. Centre National de la Recherche Scientifique

    Paris, France

    5 h-index

    19 publications

    456 citations

  4. Foundation for Applied Molecular Evolution

    Gainesville, United States

    5 h-index

    18 publications

    1.8K citations

  5. Harvard University

    Cambridge, United States

    5 h-index

    15 publications

    1.5K citations

  6. Arizona State University

    Tempe, United States

    5 h-index

    18 publications

    1.9K citations

  7. Massachusetts Institute of Technology

    Cambridge, United States

    4 h-index

    14 publications

    1.5K citations

  8. University of Konstanz

    Konstanz, Germany

    3 h-index

    7 publications

    181 citations

1–8 of 114

Venues

  1. Journal of the American Chemical Society

    JACS · 13 publications · 1.4K citations

    13

    h-index

  2. Nucleic Acids Research

    10 publications · 856 citations

    10

    h-index

  3. Angewandte Chemie International Edition

    5 publications · 326 citations

    5

    h-index

  4. Proceedings of the National Academy of Sciences

    PNAS · 4 publications · 610 citations

    4

    h-index

  5. Biochemistry

    4 publications · 131 citations

    4

    h-index

1–5