Concepedia

Double-stranded Complementary Dna Synthesis

Double-Stranded Complementary DNA Synthesis is a molecular biology technique that belongs to the class of cDNA preparation methods, specifically characterized by the enzymatic conversion of single-stranded cDNA into double-stranded DNA using DNA polymerase and RNAse H to facilitate second-strand initiation and elongation.

31

Publications

1.7K

Citations

118

Authors

44

Institutions

Publications per year

2017–2026

0

Parents

Publications

31

Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.

Marvin Wickens, Gary Buell, Robert Schimke · Journal of Biological Chemistry · 1978 · 395 citations · Full text

, , +14

Molecular cloning and sequence determination of rat preproenkephalin cDNA: sensitive probe for studying transcriptional changes in rat tissues.

Richard Howells, D L Kilpatrick, Ramesh R. Bhatt et al. · Proceedings of the National Academy of Sciences · 1984 · 191 citations · Full text

, , +14

beta zero thalassemia in Sardinia is caused by a nonsense mutation.

Richard F. Trecartin, Stephen A. Liebhaber, Judy C. Chang et al. · Journal of Clinical Investigation · 1981 · 186 citations · Full text

, , +13

Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli.

Monica J. Roth, Naoko Tanese, Stephen P. Goff · Journal of Biological Chemistry · 1985 · 166 citations · Full text

, , +17

Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.

François Rougeon, Bernard Mach · Proceedings of the National Academy of Sciences · 1976 · 72 citations · Full text

, , +15

1–5 of 31

Authors

118

  1. JJ
    John J. Monahan

    La Roche College, United States

    2 publications · 250 citations

    2

    h-index

  2. TK
    Torsten Krude

    University of Cambridge, United Kingdom

    2 publications · 37 citations

    2

    h-index

  3. RK
    Rolf Knippers

    University of Konstanz, Germany

    2 publications · 37 citations

    2

    h-index

  4. IM
    Inder M. Verma

    Salk Institute for Biological Studies, United States

    1 publications · 45 citations

    1

    h-index

  5. PF
    Paul F. Lambert

    University of Wisconsin–Madison, United States

    1 publications · 19 citations

    1

    h-index

1–5 of 118

Institutions

44

  1. University of Konstanz

    Konstanz, Germany

    2 h-index

    4 publications

    74 citations

  2. University of Geneva

    Geneva, Switzerland

    1 h-index

    2 publications

    144 citations

  3. St. Mary’s Hospital

    Durban, South Africa

    1 h-index

    1 publications

    42 citations

  4. National Cancer Institute

    Bethesda, United States

    1 h-index

    1 publications

    72 citations

  5. Institute of Bioorganic Chemistry

    Moscow, Russia

    1 h-index

    1 publications

    36 citations

  6. University of Wisconsin–Madison

    Madison, United States

    1 h-index

    4 publications

    64 citations

  7. University of Cambridge

    Cambridge, United Kingdom

    1 h-index

    2 publications

    34 citations

  8. Universidad de Málaga

    Málaga, Spain

    1 h-index

    5 publications

    210 citations

1–8 of 44

Venues

  1. Journal of Biological Chemistry

    JBC · 6 publications · 673 citations

    6

    h-index

  2. Nucleic Acids Research

    4 publications · 99 citations

    4

    h-index

  3. Proceedings of the National Academy of Sciences

    PNAS · 4 publications · 359 citations

    4

    h-index

  4. Gene

    2 publications · 35 citations

    2

    h-index

  5. 1

    h-index

1–5