Purification and characterization of a novel GTP-binding protein with a molecular weight of 24,000 from bovine brain membranes.

Akira Kikuchi, T. Yamashita, M Kawata, +4

Journal of Biological Chemistry

Molecular BiologyProtein PurificationNovel Gtp-binding ProteinProtein FoldingBovine Brain Membranes +17

A novel small molecular weight GTP-binding protein with the same putative effector domain as the ras proteins in bovine brain membranes. Purification, determination of primary structure, and characterization.

M Kawata, Yasuji Matsui, J. Kondo, +3

Journal of Biological Chemistry

C terminus of the small GTP-binding protein smg p25A contains two geranylgeranylated cysteine residues and a methyl ester.

Christopher C. Farnsworth, M Kawata, Yasuhisa Yoshida, +3

Proceedings of the National Academy of Sciences

Protein SecretionMolecular BiologyChemical BiologyCellular PhysiologyGeranylgeranylated Cysteine Residues +17

Posttranslationally processed structure of the human platelet protein smg p21B: evidence for geranylgeranylation and carboxyl methylation of the C-terminal cysteine.

M Kawata, Christopher C. Farnsworth, Yasuhisa Yoshida, +3

Proceedings of the National Academy of Sciences

ThrombopoiesisSmg P21aProtein FunctionBiochemistryBlood Platelet +11

Phosphorylation of smg p21, a ras p21-like GTP-binding Protein, by Cyclic AMP-dependent Protein Kinase in a Cell-free System and in Response to Prostaglandin E1 in Intact Human Platelets

M Kawata, Akira Kikuchi, Masahiko Hoshijima, +4

Journal of Biological Chemistry

Prostaglandin E1Cellular PhysiologyProtein Kinase ASignaling PathwayReceptor Tyrosine Kinase +18

Microinjection of smg/rap1/Krev-1 p21 into Swiss 3T3 cells induces DNA synthesis and morphological changes.

Yasuhisa Yoshida, M Kawata, Yasushi Miura, +4

Molecular and Cellular Biology

Molecular BiologyCellular PhysiologySwiss 3T3Insulin SignalingMorphological Changes +17

The geranylgeranyl moiety but not the methyl moiety of the smg-25A/rab3A protein is essential for the interactions with membrane and its inhibitory GDP/GTP exchange protein.

T Musha, M Kawata, Yoshimi Takai

Journal of Biological Chemistry

Protein FunctionSignal TransductionProtein ExpressionBiochemistrySmg-25a/rab3a Protein +13

The posttranslationally modified C-terminal structure of bovine aortic smooth muscle rhoA p21

Masahiko Katayama, M Kawata, Yasuhisa Yoshida, +4

Journal of Biological Chemistry

Molecular BiologyChemical BiologyC-terminal StructureProtein SynthesisRhoa P21 +14

A novel prenyltransferase for a small GTP-binding protein having a C-terminal Cys-Ala-Cys structure.

Hisanori Horiuchi, M Kawata, Masahiko Katayama, +4

Journal of Biological Chemistry

Molecular BiologyNovel PrenyltransferaseChemical BiologySmall MoleculesC-terminal Cys-ala-cys Structure +16

Purification and characterization of c-Ki-ras p21 from bovine brain crude membranes.

T. Yamashita, Katsuhiko Yamamoto, Akira Kikuchi, +6

Journal of Biological Chemistry