The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup

B. Bhaskar Gollapudi, Anthony M. Lynch, Robert H. Heflich, +11

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

AllergyMedicineBioanalysisInternational WorkshopLaboratory Animal Study +8

Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N-ethyl-N-nitrosourea

Daishiro Miura, Vasily N. Dobrovolsky, Takafumi Kimoto, +2

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Reactive Nitrogen SpecieMedicineSplit DosesPhysiologyHematology +4

International <i>Pig‐a</i> gene mutation assay trial: Evaluation of transferability across 14 laboratories

Stephen D. Dertinger, Souk Phonethepswath, Pamela Weller, +33

Environmental and Molecular Mutagenesis

Manifestation of Pig-a mutant bone marrow erythroids and peripheral blood erythrocytes in mice treated with N-ethyl-N-nitrosourea: Direct sequencing of Pig-a cDNA from bone marrow cells negative for GPI-anchored protein expression

Takafumi Kimoto, Kumiko Suzuki, Xiao mei Kobayashi, +4

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Bone Marrow FailureAutoimmune DiseaseLaboratory HematologyDirect SequencingGranulocyte +9

Interlaboratory trial of the rat Pig-a mutation assay using an erythroid marker HIS49 antibody

Takafumi Kimoto, Katsuyoshi Horibata, Satsuki Chikura, +7

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

HistocompatibilityAnimal TestingAutoimmune DiseaseMedicineHistopathology +9

Further development of the rat <i>Pig‐a</i> mutation assay: Measuring rat <i>Pig‐a</i> mutant bone marrow erythroids and a high throughput assay for mutant peripheral blood reticulocytes

Takafumi Kimoto, Satsuki Chikura, Kumiko Suzuki, +8

Environmental and Molecular Mutagenesis

ImmunohematologyImmunologyHigh ThroughputBlood CellEmbryology +15

The PIGRET assay, a method for measuring Pig-a gene mutation in reticulocytes, is reliable as a short-term in vivo genotoxicity test: Summary of the MMS/JEMS-collaborative study across 16 laboratories using 24 chemicals

Takafumi Kimoto, Katsuyoshi Horibata, Daishiro Miura, +31

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Rosa26-GFP Direct Repeat (RaDR-GFP) Mice Reveal Tissue- and Age-Dependence of Homologous Recombination in Mammals &lt;i&gt;In Vivo&lt;/i&gt;

Michelle R. Sukup-Jackson, Orsolya Király, Jennifer E. Kay, +14

Figshare

Flow cytometric detection of <i>Pig‐A</i> mutant red blood cells using an erythroid‐specific antibody: Application of the method for evaluating the in vivo genotoxicity of methylphenidate in adolescent rats

Vasily N. Dobrovolsky, Sherin Y. Boctor, Nathan C. Twaddle, +7

Environmental and Molecular Mutagenesis

Evaluation of <i>in vivo</i> genotoxicity induced by <i>N</i>‐ethyl‐<i>N</i>‐nitrosourea, benzo[<i>a</i>]pyrene, and 4‐nitroquinoline‐1‐oxide in the <i>Pig‐a</i> and <i>gpt</i> assays

Katsuyoshi Horibata, Akiko Ukai, Takafumi Kimoto, +5

Environmental and Molecular Mutagenesis