David R. Higgins

Mutation of lysine-48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP.

Patrick Sung, David R. Higgins, Louise Prakash et al. · The EMBO Journal · 1988 · 264 citations · Full text

Cellular Enzymology, Biochemistry, Natural Sciences +6

Introduction to Pichia pastoris

David R. Higgins, James M. Cregg · Methods in molecular biology · 1998 · 191 citations

[21] Recovery of plasmids from yeast into Escherichia coli: Shuttle vectors

Jeffrey N. Strathern, David R. Higgins · Methods in enzymology on CD-ROM/Methods in enzymology · 1991 · 145 citations

Engineering, Microbial Genetics, Biotechnology +9

Isolation and characterization of the <i>RAD3</i> gene of <i>Saccharomyces cerevisiae</i> and inviability of <i>rad3</i> deletion mutants

David R. Higgins, Satya Prakash, Paul R. Reynolds et al. · Proceedings of the National Academy of Sciences · 1983 · 108 citations · Full text

Production of foreign proteins in the yeast <i>Pichia pastoris</i>

James M. Cregg, David R. Higgins · Canadian Journal of Botany · 1995 · 93 citations

Engineering, Molecular Biology, Protein Synthesis +20