Simultaneous Absolute Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases as a Novel Approach for the Characterization of Individual Human Liver: Comparison with mRNA Levels and Activities

Sumio Ohtsuki, Olaf Schaefer, Hirotaka Kawakami, +8

Drug Metabolism and Disposition

Quantitative Membrane Protein Expression at the Blood–Brain Barrier of Adult and Younger Cynomolgus Monkeys

Katsuaki Ito, Yasuo Uchida, Sumio Ohtsuki, +5

Journal of Pharmaceutical Sciences

Molecular NeuroscienceMolecular PhysiologySynaptic SignalingBlood–brain BarrierNeuroanatomy +12

Simultaneous Absolute Quantification of 11 Cytochrome P450 Isoforms in Human Liver Microsomes by Liquid Chromatography Tandem Mass Spectrometry with In Silico Target Peptide Selection

Hirotaka Kawakami, Sumio Ohtsuki, Junichi Kamiie, +3

Journal of Pharmaceutical Sciences

BiochemistryGas ChromatographyMedicineLiver PhysiologySimultaneous Absolute Quantification +11

Absolute Quantification and Differential Expression of Drug Transporters, Cytochrome P450 Enzymes, and UDP-Glucuronosyltransferases in Cultured Primary Human Hepatocytes

Olaf Schaefer, Sumio Ohtsuki, Hirotaka Kawakami, +8

Drug Metabolism and Disposition

Proteomics Reveals N-Linked Glycoprotein Diversity in Caenorhabditis elegans and Suggests an Atypical Translocation Mechanism for Integral Membrane Proteins

Hiroyuki Kaji, Junichi Kamiie, Hirotaka Kawakami, +6

Molecular & Cellular Proteomics

Human Platelets Express Organic Anion-Transporting Peptide 2B1, an Uptake Transporter for Atorvastatin

Gabriele Jedlitschky, Markus Grube, Sandra Bien, +12

Drug Metabolism and Disposition

Reliability and Robustness of Simultaneous Absolute Quantification of Drug Transporters, Cytochrome P450 Enzymes, and Udp-Glucuronosyltransferases in Human Liver Tissue by Multiplexed MRM/Selected Reaction Monitoring Mode Tandem Mass Spectrometry with Nano-Liquid Chromatography

Atsushi Sakamoto, Takehisa Matsumaru, Naoki Ishiguro, +5

Journal of Pharmaceutical Sciences

BiochemistryMedicineLiver PhysiologyBioanalysisMass Spectrometry +9

Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals

Hirofumi Kaneko, Junichi Kamiie, Hirotaka Kawakami, +7

Analytical Biochemistry

Protein ChemistryProteomic TechnologyProtein AssemblyBiochemistryProteome Analysis +5

Osmium Impregnation of Methyl Methacrylate Vascular Casts for Scanning Electron Microscopy

Takuro MURAKAMI, Magosaburo UNEHIRA, Hirotaka Kawakami, +1

Archivum histologicum japonicum

MicroscopyBiomedical EngineeringChemistryRenal FunctionElectron Microscopy +15

Validation of uPA/SCID Mouse with Humanized Liver as a Human Liver Model: Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases by LC-MS/MS

Sumio Ohtsuki, Hirotaka Kawakami, Tae Inoue, +8

Drug Metabolism and Disposition