Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy

Michael Marko, Chyongere Hsieh, Richard Schalek, +2

Nature Methods

EngineeringElectron MicroscopyMicroscopyMicroscopy MethodBiomedical Imaging +5

Towards high-resolution three-dimensional imaging of native mammalian tissue: Electron tomography of frozen-hydrated rat liver sections

Chyongere Hsieh, A. Leith, Carmen A. Mannella, +2

Journal of Structural Biology

Computed TomographyTissue ImagingRadiologyEngineeringMedical Imaging +11

A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution

Bruce F. McEwen, Chyongere Hsieh, Alexa L. Mattheyses, +1

Chromosoma

Cell PhysiologyCell DivisionNatural SciencesBiophysical AspectMolecular Biology +11

Electron tomographic analysis of frozen-hydrated tissue sections

Chyongere Hsieh, Michael Marko, Joachim Frank, +1

Journal of Structural Biology

Medical ImagingMicroscopyBiomedical ImagingElectron MicroscopeElectron Tomographic Analysis +5

Cryo-Electron Tomography Reveals the Comparative Three-Dimensional Architecture of<i>Prochlorococcus</i>, a Globally Important Marine Cyanobacterium

Claire S. Ting, Chyongere Hsieh, Sesh A. Sundararaman, +2

Journal of Bacteriology

Cryo-Electron Tomography Elucidates the Molecular Architecture of<i>Treponema pallidum</i>, the Syphilis Spirochete

Jacques Izard, Christian Renken, Chyongere Hsieh, +8

Journal of Bacteriology

The Flat-Ribbon Configuration of the Periplasmic Flagella of <i>Borrelia burgdorferi</i> and Its Relationship to Motility and Morphology

Nyles W. Charon, Stuart F. Goldstein, Michael Marko, +6

Journal of Bacteriology

MicroscopyBacteriologyBacteriophageMolecular BiologyTick-borne Disease +15

Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography

Chyongere Hsieh, Thomas Schmelzer, Gregory Kishchenko, +2

Journal of Structural Biology

Cryo-tem TomographyTissue ImagingPractical WorkflowEngineeringMedical Imaging +8

Structural Evidence of Glycoprotein Assembly in Cellular Membrane Compartments prior to Alphavirus Budding

Pan Soonsawad, Xing Li, Emerson Milla, +11

Journal of Virology

Viral ReplicationMolecular BiologyCytoskeletonViral Structural ProteinVirus Structure +14

Use of frozen-hydrated axonemes to assess imaging parameters and resolution limits in cryoelectron tomography

Bruce F. McEwen, Michael Marko, Chyongere Hsieh, +1

Journal of Structural Biology

Tissue ImagingEngineeringMedical ImagingMicroscopyNovel Imaging Method +11