23:T426,Tomato yellow leaf curl virus (TYLCV) poses serious threat to tomato production worldwide, and the vector, Bemisia tabaci, plays a key role in the transmission of this virus. However, the molecular mechanisms underlying the transmission remain poorly understood. In this study, firstly, we identified the whitefly proteins that presumably interact with TYLCV coat protein (CP) using split-ubiquitin yeast two-hybrid system. Next, we conducted GST pull-down and immunofluorescence to examine the potential interaction between TYLCV CP and one of the proteins identified, namely vesicle associated membrane protein-associated protein B (VAPB), an protein abundantly expressed in whitefly midgut. Further experiments demonstrated that VAPB was significantly up-regulated upon virus acquisition, and silencing VAPB led to a significant increase of relative virus quantity in whitefly haemolymph and salivary glands, as well as an increase of TYLCV transmission efficiency. These findings indicate an important role of VAPB in the transmission of TYLCV by whiteflies.24:T54e,Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the Bemisia tabaci complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of Vta1 in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly.25:T4cc,Begomoviruses cause substantial losses to agricultural production, especially in tropical and subtropical regions, and are exclusively transmitted by members of the whitefly Bemisia tabaci species complex. However, the molecular mechanisms underlying the transmission of begomoviruses by their whitefly vector are not clear. In this study, we found that B. tabaci vesicle-associated membrane protein 2 (BtVAMP2) interacts with the coat protein (CP) of tomato yellow leaf curl virus (TYLCV), an emergent begomovirus that seriously impacts tomato production globally. After infection with TYLCV, the transcription of BtVAMP2 was increased. When the BtVAMP2 protein was blocked by feeding with a specific BtVAMP2 antibody, the quantity of TYLCV in B. tabaci whole body was significantly reduced. BtVAMP2 was found to be conserved among the B. tabaci species complex and also interacts with the CP of Sri Lankan cassava mosaic virus (SLCMV). When feeding with BtVAMP2 antibody, the acquisition quantity of SLCMV in whitefly whole body was also decreased significantly. Overall, our results demonstrate that BtVAMP2 interacts with the CP of begomoviruses and promotes their acquisition by whitefly.

#	Concept	H-Index	Publications	Citations
1	Medicine	2	2	71
2	Biology	2	2	71
3	Microbiology	2	2	71
4	Pathogenesis	2	2	71
5	Entomology	1	1	47

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