Masao Ohyama

High-level expression of recombinant human serum albumin from the methylotrophic yeast Pichia pastoris with minimal protease production and activation

Kaoru Kobayashi, Shinobu Kuwae, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2000 · 196 citations

Minimal Protease Production, Biosynthesis, Engineering +11

High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy

Kaoru Kobayashi, Shinobu Kuwae, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2000 · 89 citations

Biomanufacturing, Engineering, Food Fermentation +12

Optimization of human serum albumin production in methylotrophic yeast <i>Pichia pastoris</i> by repeated fed‐batch fermentation

Tomoshi Ohya, Masao Ohyama, Kaoru Kobayashi · Biotechnology and Bioengineering · 2005 · 44 citations

Addition of oleic acid increases expression of recombinant human serum albumin by the AOX2 promoter in Pichia pastoris

Kaoru Kobayashi, Shinobu Kuwae, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2000 · 31 citations

Biosynthesis, Aox2 Promoter, Engineering +6

Production of recombinant human antithrombin by Pichia pastoris

Shinobu Kuwae, Masao Ohyama, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2005 · 17 citations

Biosynthesis, Biomanufacturing, Engineering +6