The aim of this study was to examine the acute effect of aerobic exercise when performed in the morning and evening on obesity-related hormones of asprosin, spexin, lipocalin-2, and insulin in normal weight (NW) and overweight/obese (OW/OO) adults. A total of 20 adult male individuals (10 NW and 10 OW/OO) volunteered their participation. Both groups were subjected to an aerobic exercise protocol in moderate intensity (heart rate reserve of 55-59%) for 30 min at two different time periods of the day (morning: 08:00-10:00 h, evening: 20.00-22.00 h) at least 3 d apart. BeBis analysis revealed the OW/OO group consumed significantly less energy (1781.59 ± 410.71 kcal) as compared with NW group (2380.28 ± 445.50 kcal) before the evening exercise (about 3 d) (p <.05). As compared with the NW group, basal serum asprosin, insulin, and lipocalin-2 hormone levels were higher in the OW/OO group, and serum spexin level was lower in OW/OO group (p <.05). Body temperature significantly increased after morning and evening aerobic exercise in both groups. The increase in body temperature was significantly higher after the evening exercise in the OW/OO group compared to the NW group (p <.05). Significant decrease in serum asprosin lipocalin-2, and insulin levels was observed in both groups after exercise (p <.05). Evening aerobic exercise more greatly decreased serum asprosin, lipocalin-2, and insulin level in the OW/OO group as compared with the NW group (p <.05). In conclusion, it is thought that negative energy balance caused by psychological energy restriction and evening aerobic exercise, which leads to a further increase in body temperature, triggers greater decrease of orexigenic signals (suppression of appetite), and is more effective in the development of adipose tissue inflammation and insulin sensitivity, especially in OW/OO group.

#	Concept	H-Index	Publications	Citations
1	Economics	1	1	12
2	Performance Studies	1	1	12

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