Fungi of the family Botryosphaeriaceae are considered responsible for various symptoms in avocado such as dieback, external necrosis of branches and inflorescences, cankers on branches and trunks, or stem-end rot of fruits. In recent years, these problems are becoming more frequent in avocado orchards in the Canary Islands (Spain). This work includes the characterization of fungal species involved in these diseases, which were isolated from avocado crops in Tenerife Island between 2018 and 2022. A total of 158 vegetal samples were collected, from which 297 fungal isolates were culture-isolated. Fifty-two of them were selected according to their morphological features as representative isolates of Botryosphaeriaceae, and their molecular characterization was carried out, sequencing the ITS1-2 region as well as the β-tubulin and the elongation factor 1-alpha genes. Five species of Botryosphaeriaceae were isolated, including Neofusicoccum australe, N. cryptoaustrale/stellenboschiana, N. luteum, N. parvum, and Lasiodiplodia brasiliensis. This is the first time that L. brasiliensis has been associated with avocado dieback and that N. cryptoaustrale/stellenboschiana has been cited in avocado causing symptoms of dieback and stem-end rot. However, it was not possible to assign our isolates unequivocally to N. cryptoaustrale or N. stellenboschiana even additionally using the rpb2 marker for their molecular characterization. Botryosphaeriaceae family seem to be involved in avocado dieback, in the premature fall of fruits during their development in the field and in post-harvest damage in Tenerife, but further studies are needed to clarify the fungal pathogens associated with symptoms in relation to phenological plant growth stages or less frequently observed.

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