23:T4d4,In recent years, it is becoming increasingly evident that once nanoparticles come into contact with biological fluids, a protein corona surely forms and critically affects the biological behaviors of nanoparticles. Herein, we investigate whether the formation of protein corona on the surface of superparamagnetic iron oxides (SPIOs) is influenced by static magnetic field. Under static magnetic field, there is no obvious variation in the total amount of protein adsorption, but the proportion of adsorbed proteins significantly changes. Noticeably, certain proteins including apolipoproteins, complement system proteins and acute phase proteins, increase in the protein corona of SPIOs in the magnetic field. More importantly, the magnetic-dependent protein corona of SPIOs enhances the cellular uptake of SPIOs into the normal cell line (3T3 cells) and tumor cell line (HepG2 cells), due to increased adsorption of apolipoprotein. In addition, SPIOs with the magnetic-dependent protein corona cause high cytotoxicity to 3T3 cells and HepG2 cells. This work discloses that superparamagnetism as a key feature of SPIOs affects the composition of protein corona to a large extent, which further alters the biological behaviors of SPIOs.24:T548,Glycoproteins are related to many biological activities and diseases and their effective capture and release from a complex mixture is of great significance in proteomics and diagnostics. However, to separate and target glycoproteins with high efficiency and selectivity is difficult, due to the interference of nanomaterials and other proteins in complicated samples. In this study, Fe₃O₄/carboxymethylated chitosan/poly(3-acrylaminophenylboronic acid) (Fe₃O₄/CMCS/PAAPBA) nanospheres with enhanced properties are prepared by a facile and economical method, and possess excellent morphology, uniform size (∼300 nm), high saturation magnetization (59 emu g^-1), and high magnetic content (79%). Due to the introduction of PAAPBA, the Fe₃O₄/CMCS/PAAPBA nanospheres have been developed as a pH-responsive strategy to reversibly capture and release glycoproteins with high selectivity and recyclability in a pure protein, a model protein mixture and even a biological sample. Additionally, the Fe₃O₄/CMCS/PAAPBA nanospheres retained high separation efficiency (∼94%) in a pure protein system after being recycled five times, showing the great potential of the boronate functionalized, pH-stimuli-responsive, magnetic nanospheres in the biomedical field.25:T5ae,

#	Concept	H-Index	Publications	Citations
1	Engineering	7	7	313
2	Medicine	4	4	192
3	Natural Sciences	1	1	34
4	Applied Physics	1	1	13
5	Biochemistry	1	1	34

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