Fruits are safe, toxin-free, and biomolecule-rich raw materials that may be utilized to decrease metal ions and stabilize nanoparticles. Here, we demonstrate the green synthesis of magnetite nanoparticles which were first capped with a layer of silica, followed by the decoration of silver nanoparticles, termed Ag@SiO₂@Fe₃O₄, by using lemon fruit extract as the reducing agent in a size range of ∼90 nm. The effect of the green stabilizer on the characteristics of nanoparticles was examined via different spectroscopy techniques, and the elemental composition of the multilayer-coated structures was verified. The saturation magnetization of bare Fe₃O₄ nanoparticles at room temperature was recorded as 78.5 emu/g, whereas it decreased to 56.4 and 43.8 emu/g for silica coating and subsequent decoration with silver nanoparticles. All nanoparticles displayed superparamagnetic behavior with almost zero coercivity. While magnetization decreased with further coating processes, the specific surface area increased with silica coating from 67 to 180 m² g^-1 and decreased after the addition of silver and reached 98 m² g^-1, which can be explained by the organization of silver nanoparticles in an island-like model. Zeta potential values also decreased from -18 to -34 mV with coating, indicating an enhanced stabilization effect of the addition of silica and silver. The antibacterial tests against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) revealed that the bare Fe₃O₄ and SiO₂@Fe₃O₄ did not show sufficient effect, while Ag@SiO₂@Fe₃O₄, even at low concentrations (≤ 200 μg/mL), displayed high antibacterial activity due to the existence of silver atoms on the surface of nanoparticles. Furthermore, the in vitro cytotoxicity assay revealed that Ag@SiO₂@Fe₃O₄ nanoparticles were not toxic to HSF-1184 cells at 200 μg/mL concentration. Antibacterial activity during consecutive magnetic separation and recycling steps was also investigated, and nanoparticles offered a high antibacterial effect for more than 10 cycles of recycling, making them potentially useful in biomedical fields.

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