Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

Mark D. Szczelkun, Maria S. Tikhomirova, Tomas Šinkūnas, +5

Proceedings of the National Academy of Sciences

EngineeringMolecular BiologyDistal Protospacer EndCascade Effector ComplexesSingle Rna-guided Cas9 +18

Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease

Tautvydas Karvelis, Gytis Druteika, Greta Bigelyte, +6

Nature

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

Tautvydas Karvelis, Greta Bigelyte, Joshua K. Young, +9

Nucleic Acids Research

Pam RecognitionOff-target EffectEngineeringSynthetic BiologyMolecular Biology +11

crRNA and tracrRNA guide Cas9-mediated DNA interference in<i>Streptococcus thermophilus</i>

Tautvydas Karvelis, Giedrius Gasiūnas, Algirdas Mikšys, +3

RNA Biology

EngineeringMicrobial PathogensMinimal Crispr ArrayBacteriologyMolecular Biology +17

Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

Tautvydas Karvelis, Giedrius Gasiūnas, Joshua K. Young, +4

Genome biology

A catalogue of biochemically diverse CRISPR-Cas9 orthologs

Giedrius Gasiūnas, Joshua K. Young, Tautvydas Karvelis, +22

Nature Communications

Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells

Greta Bigelyte, Joshua K. Young, Tautvydas Karvelis, +17

Nature Communications

An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis

T Hino, Satoshi N. Omura, Ryoya Nakagawa, +24

Cell

EngineeringIn Vivo Gene TherapyGeneticsMolecular BiologyGenomics +19

TnpB structure reveals minimal functional core of Cas12 nuclease family

Giedrius Sasnauskas, Giedrė Tamulaitienė, Gytis Druteika, +7

Nature

Systems BiologyStructural BioinformaticsMolecular BiologyStructural GenomicsMolecular Research +1

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

Grace Mullally, Kara van Aelst, Mohsin M. Naqvi, +5

Nucleic Acids Research