1b:I[76749,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 1c:I[3486,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 1d:I[41892,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 1e:I[17707,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 1f:I[66528,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 20:I[63176,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 21:I[87099,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 22:I[26200,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"Group"] 23:I[23365,["/_next/static/chunks/f9f65a69b09cd00d.js","/_next/static/chunks/1ba21a8e081e29d1.js","/_next/static/chunks/0383663887945ae7.js","/_next/static/chunks/5bf36002c846d2d8.js","/_next/static/chunks/63561dcd578bd4db.js","/_next/static/chunks/29d2646804fd1cab.js","/_next/static/chunks/2060b26370dc6c0a.js"],"default"] 24:T431,The 3-O-sulfated glucosamine in heparan sulfate (HS) is a low-abundance structural component, but it is a key saccharide unit for the biological activities of HS. A method to determine the level of 3-O-sulfated HS is lacking. Here, we describe a LC-MS/MS based method to analyze the structural motifs. We determined the levels of 3-O-sulfated structural motifs from pharmaceutical heparin manufactured from bovine, porcine, and ovine. We discovered that saccharide chains carrying 3-O-sulfation from enoxaparin, an FDA-approved low-molecular weight heparin, displayed a slower clearance rate than non-3-O-sulfated sugar chains in a mouse model. Lastly, we detected the 3-O-sulfated HS from human brain. Furthermore, we found that a specific 3-O-sulfated structural motif, tetra-1, is elevated in the brain HS from Alzheimer's disease patients (n = 5, p = 0.0020). Our method offers a practical solution to measure 3-O-sulfated HS from biological sources with the sensitivity and quantitative capability.

Vijay M. Dhurandahare

Publications