Toyoo Ohda

High-level expression of recombinant human serum albumin from the methylotrophic yeast Pichia pastoris with minimal protease production and activation

Kaoru Kobayashi, Shinobu Kuwae, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2000 · 196 citations

Minimal Protease Production, Biosynthesis, Engineering +11

High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy

Kaoru Kobayashi, Shinobu Kuwae, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2000 · 89 citations

Biomanufacturing, Engineering, Food Fermentation +12

Development of a fed-batch culture process for enhanced production of recombinant human antithrombin by Chinese hamster ovary cells

Shinobu Kuwae, Toyoo Ohda, Hiroshi Tamashima et al. · Journal of Bioscience and Bioengineering · 2005 · 50 citations

Biomanufacturing, Xenotransplantation, Recombinant Human Antithrombin +9

Analysis of <i>Pichia pastoris</i> Components in Recombinant Human Serum Albumin by Immunological Assays and by HPLC with Pulsed Amperometric Detection

Wataru Ohtani, Toyoo Ohda, Akinori Sumi et al. · Analytical Chemistry · 1998 · 44 citations

Engineering, Glycobiology, Polysaccharide +19

Addition of oleic acid increases expression of recombinant human serum albumin by the AOX2 promoter in Pichia pastoris

Kaoru Kobayashi, Shinobu Kuwae, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2000 · 31 citations

Biosynthesis, Aox2 Promoter, Engineering +6