Holger Puchta

Both <scp>CRISPR</scp>/<scp>C</scp>as‐based nucleases and nickases can be used efficiently for genome engineering in <i><scp>A</scp>rabidopsis thaliana</i>

Friedrich Fauser, Simon Schiml, Holger Puchta · The Plant Journal · 2014 · 720 citations · Full text

Engineering, Genetics, Cas9 Nickase +13

Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination.

Holger Puchta, Bernard Dujon, Barbara Höhn · Proceedings of the National Academy of Sciences · 1996 · 426 citations · Full text

Biology, Plant Molecular Biology, Genome Instability +14

Elevated UV-B radiation reduces genome stability in plants

Gerhard Ries, Werner Heller, Holger Puchta et al. · Nature · 2000 · 404 citations · Full text

Biology, Genome Stability, Plant Genetics +5

The <scp>CRISPR</scp>/<scp>C</scp>as system can be used as nuclease for <i>in planta</i> gene targeting and as paired nickases for directed mutagenesis in <scp>A</scp>rabidopsis resulting in heritable progeny

Simon Schiml, Friedrich Fauser, Holger Puchta · The Plant Journal · 2014 · 343 citations

Homologous recombination in plant cells is enhanced by<i>in vivo</i>induction of double strand breaks into DNA by a site-specific endonuclease

Holger Puchta, Bernard Dujon, Barbara Höhn · Nucleic Acids Research · 1993 · 287 citations · Full text