21:I[432972,["/_next/static/chunks/bb923814e1f4d2e9.js","/_next/static/chunks/dd3ee9f5b7716ca6.js","/_next/static/chunks/4204a0ae82542ca8.js","/_next/static/chunks/1270dead247da766.js","/_next/static/chunks/6e1aa89a642f40be.js","/_next/static/chunks/2d7aa753e81324a8.js","/_next/static/chunks/8ffd0b71567920d8.js","/_next/static/chunks/67ea6dfd21f16c5f.js","/_next/static/chunks/24eb4379f68013fd.js","/_next/static/chunks/b8cd775ffdf935bd.js","/_next/static/chunks/983103cd9eb7b428.js","/_next/static/chunks/263f2cdcb9aa2341.js","/_next/static/chunks/294b9525248c8e5f.js"],"default"] 22:T418,The primary culture of rabbit corneal endothelium after isolation by trypsinization has been reported.¹Although this technique was uniformly successful in rabbits and monkeys, identical experiments upon human eyes failed with rare exception. A modification in technique has afforded success in 18 consecutive experiments on human corneas and, therefore, is thought worthy of reporting. This paper describes the modified technique for isolation and the primary growth of human corneal endothelium in tissue culture and presents observations of cell activity and the appearance of the endothelial mitochondria. A brief summary of the isolation and growth techniques which were unsuccessful is also reported.

Materials and Methods

The studies utilized human eyes which were not required for corneal transplantation. The eyes, varying from 28 to 70 years-of-age, were removed under surgically aseptic conditions within six hours after death, and washed with 0.5% ophthalmic Neosporin* solution. Cell culture was established 24 to 7223:T405,

Introduction

The present study concerns the isolation of rabbit corneal endothelial cells and their culture in sufficient number for experimental studies. During the last half of the 19th century flat preparations of corneal endothelium were first made. Later, Nagano separated Descemet's membrane with its endothelial covering.¹In 1929, Matsui cultured rabbit corneal endothelial cells that had been isolated by stripping Descemet's membrane.²Recently, Stocker et al devised a technique of mechanically separating the cornea into endothelial, stromal, and epithelial layers for use in tissue culture.³ This paper describes the use of an enzyme to isolate corneal endothelial cells, and presents observations on the activities of these cells in tissue culture.

Materials and Methods

The present study was conducted on adult albino and pigmented rabbits of 1.5 to 2 kg body weight. The animals were killed by intravenous air injection, the eyes enucleated and placed in a sterile

#	Concept	H-Index	Publications	Citations
1	Medicine	4	4	134
2	Cell Biology	2	2	81
3	Engineering	1	1	21
4	Biomedical Engineering	1	1	21
5	Cellular Physiology	1	1	13

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