Shigefumi Kumachi

Construction of a Humanized Artificial VHH Library Reproducing Structural Features of Camelid VHHs for Therapeutics

Taihei Murakami, Shigefumi Kumachi, Yasuhiro Matsunaga et al. · Antibodies · 2022 · 29 citations · Full text

Protein Assembly, Structural Bioinformatics, Biomolecular Structure Prediction +20

An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display

Shigefumi Kumachi, Yuzuru Husimi, Naoto Nemoto · ACS Omega · 2016 · 19 citations · Full text

In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR

Chathuni Jayathilake, Shigefumi Kumachi, Hidenao Arai et al. · Analytical Biochemistry · 2019 · 15 citations

Immunocytochemical Technique, Allergy, Autoimmune Disease +13

In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display

Naoto Nemoto, Shigefumi Kumachi, Hidenao Arai · Methods in molecular biology · 2018 · 13 citations

Immunocytochemical Technique, Cdna Display, Allergy +6

Versatile C-Terminal Specific Biotinylation of Proteins Using Both a Puromycin-Linker and a Cell-Free Translation System for Studying High-Throughput Protein–Molecule Interactions

Naoto Nemoto, Takayuki Fukushima, Shigefumi Kumachi et al. · Analytical Chemistry · 2014 · 11 citations

Immunocytochemical Technique, Molecular Biology, High-throughput Protein–molecule Interactions +20