David Shaw

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA

Soyoung Oh, Kate Senger, Shravan Madireddi et al. · The Journal of Experimental Medicine · 2022 · 79 citations · Full text

Engineering, Immunology, Immune Cell Therapy +19

Maximizing antibody production in a targeted integration host by optimization of subunit gene dosage and position

Joe Carver, Domingos Ng, Michelle Zhou et al. · Biotechnology Progress · 2020 · 60 citations

Full Sequencing of CRISPR/Cas9 Single Guide RNA (sgRNA) via Parallel Ribonuclease Digestions and Hydrophilic Interaction Liquid Chromatography–High-Resolution Mass Spectrometry Analysis

Alexandre Goyon, Brandon L. Scott, Kenji L. Kurita et al. · Analytical Chemistry · 2021 · 58 citations · Full text

Parallel Ribonuclease Digestions, Rnase Digestion Products, Functional Genomics +14

Probing the importance of clonality: Single cell subcloning of clonally derived CHO cell lines yields widely diverse clones differing in growth, productivity, and product quality

Peggy Ko, Shahram Misaghi, Zhilan Hu et al. · Biotechnology Progress · 2017 · 50 citations

Immunocytochemical Technique, Genetics, Immunology +17

FX knockout CHO hosts can express desired ratios of fucosylated or afucosylated antibodies with high titers and comparable product quality

Salina Louie, Benjamin Haley, Brett Marshall et al. · Biotechnology and Bioengineering · 2016 · 45 citations

Immunology, Immunologic Mechanism, Afucosylated Antibodies +20