Joseph A. Affholter

Molecular evolution by staggered extension process (StEP) in vitro recombination

Huimin Zhao, Lori Giver, Zhixin Shao et al. · Nature Biotechnology · 1998 · 755 citations

Molecular Biotechnology, Engineering, Directed Evolution +10

Haloalkane Dehalogenases:  Structure of a<i>Rhodococcus</i>Enzyme^,

Janet Newman, Thomas S. Peat, R. Richard et al. · Biochemistry · 1999 · 158 citations

Human Insulin-Degrading Enzyme Shares Structural and Functional Homologies with <i>E. coli</i> Protease III

Joseph A. Affholter, Victor A. Fried, Richard A. Roth · Science · 1988 · 147 citations

Escherichia Coli Proteinase, Protein Assembly, Proteasome +18

Insulin-Degrading Enzyme: Stable Expression of the Human Complementary DNA, Characterization of its Protein Product, and Chromosomal Mapping of the Human and Mouse Genes

Joseph A. Affholter, Chih‐Lin Hsieh, Uta Francke et al. · Molecular Endocrinology · 1990 · 70 citations · Full text

Design, structure-activity, and molecular modeling studies of potent renin inhibitory peptides having N-terminal Nin-For-Trp (Ftr): angiotensinogen congeners modified by P1-P1' Phe-Phe, Sta, Leu.psi.[CH(OH)CH2]Val or Leu.psi.[CH2NH]Val substitutions

Tomi K. Sawyer, Donald T. Pals, Boryeu Mao et al. · Journal of Medicinal Chemistry · 1988 · 37 citations · Full text